#config file used to map bisulphite seq data. Conver to .yaml format if using with pipeline THREADS: 4 FILTER: # adapter: AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC # extra_params_trim: ' -a "AAAAAAA -a TTTTTTT " --clip_R2 10 --clip_R1 10 --phred33 --length 20 ' extra_params_trim: " -a T{20} -a2 A{20} --phred33 --length 20 " # example for trimming 10bp off 5' # extra_params_trim: --clip_R2 10 --clip_R1 10 MAPPING: # this folder must contain the bismark index, i.e. a folder "Bisulfite_Genome" # along with the fa file it was build from (e.g. chloroplast.fa) reference: "/data/public_data/tomato/S_lycopersicum/plusChloroplast/" # used as short version to name output filnames for mapping # this should ideally be the filename (e.g. chloroplast.fa) without .fa extension reference_short: "S_lycopersicum_chromosomes.3.00_plusChloroplast" extra_params_bismark: " --dovetail --score_min L,0,-0.4 -un" # example to relax mapping # extra_params_bismark: " --score_min L,0,-0.4 --un" REPORT: binsize: 50 extra_params_bamCoverage: "" extra_params_meth_extractor: " --comprehensive --report --buffer_size 8G" extra_params_bismark2bedGraph: "" extra_params_coverage2cytosine: " --CX"